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cynomolgus cd3 epsilon protein  (ACROBiosystems)


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    Structured Review

    ACROBiosystems cynomolgus cd3 epsilon protein
    Treg suppression assay Measurement of CTV dilution after co-culture between Tregs, CTV labeled PBMCs, and <t>α-CD3/α-CD28</t> beads to assess Treg suppressor ability.
    Cynomolgus Cd3 Epsilon Protein, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cynomolgus+cd3+epsilon+protein/Cynomolgus+CD3+epsilon+Protein%2C+Fc%2CHis+Tag/pmc09641266-16-0-7
    Average 90 stars, based on 1 article reviews
    cynomolgus cd3 epsilon protein - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation"

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101784

    Treg suppression assay Measurement of CTV dilution after co-culture between Tregs, CTV labeled PBMCs, and α-CD3/α-CD28 beads to assess Treg suppressor ability.
    Figure Legend Snippet: Treg suppression assay Measurement of CTV dilution after co-culture between Tregs, CTV labeled PBMCs, and α-CD3/α-CD28 beads to assess Treg suppressor ability.

    Techniques Used: Suppression Assay, Co-Culture Assay, Labeling


    Figure Legend Snippet:

    Techniques Used: Staining, Recombinant, Activation Assay, Plasmid Preparation, Purification, Synthesized, Expressing, Clone Assay, Software, Irradiation

    Related Articles

    Suppression Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Co-Culture Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Labeling:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Staining:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Recombinant:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Activation Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Plasmid Preparation:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Purification:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Synthesized:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Expressing:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Clone Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Software:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Irradiation:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Cell Culture:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Transduction:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Incubation:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Flow Cytometry:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Binding Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Control:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    In Vitro:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Isolation:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Methylation:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Methylation Sequencing:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Adoptive Transfer Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
    Article Snippet: CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.CD25-PE/Dazzle 594 (1:100 dilution for extracellular only staining, 1:20 dilution if performing intracellular staining) , BioLegend , Cat#302646; Clone: BC96; RRID: AB_2734260.. Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.. His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.His Tag-AF647 (1:100 dilution) , BioLegend , Cat#652513; Clone: J099B12; RRID: AB_2716153.

    Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque
    Article Snippet: Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.Streptavidin-PE , BD Biosciences , Cat#554061; RRID: AB_10053328.. Cynomolgus CD3 epsilon protein , ACRO Biosystems , Cat#CDE-C5254.. Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.Biotinylated Human CD3 epsilon protein , ACRO Biosystems , Cat#CDE-H82E1.



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    Image Search Results


    Treg suppression assay Measurement of CTV dilution after co-culture between Tregs, CTV labeled PBMCs, and α-CD3/α-CD28 beads to assess Treg suppressor ability.

    Journal: STAR Protocols

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

    doi: 10.1016/j.xpro.2022.101784

    Figure Lengend Snippet: Treg suppression assay Measurement of CTV dilution after co-culture between Tregs, CTV labeled PBMCs, and α-CD3/α-CD28 beads to assess Treg suppressor ability.

    Article Snippet: Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.

    Techniques: Suppression Assay, Co-Culture Assay, Labeling

    Journal: STAR Protocols

    Article Title: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

    doi: 10.1016/j.xpro.2022.101784

    Figure Lengend Snippet:

    Article Snippet: Cynomolgus CD3 epsilon protein (1:100 dilution) , ACROBiosystems , Cat#CDE-C5526.

    Techniques: Staining, Recombinant, Activation Assay, Plasmid Preparation, Purification, Synthesized, Expressing, Clone Assay, Software, Irradiation